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male cd1 mice  (Inotiv)


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    Structured Review

    Inotiv male cd1 mice
    Male Cd1 Mice, supplied by Inotiv, used in various techniques. Bioz Stars score: 95/100, based on 1615 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/icr+mice/ICR+Mouse/10__1021_slash_acsptsci__6c00453-16-0-5
    Average 95 stars, based on 1615 article reviews
    male cd1 mice - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    In Vivo:

    Article Title: Development of mRNA–lipid nanoparticle intrabodies against rickettsial infection
    Article Snippet: HEK293 cells (2 × 10 6 cells) were transfected with 2 μg of mRNA-LNPs encoding A44, A123, or A171 or with an equal volume of PBS as a negative control for 6 h. The cells were then infected with host cell–free E. chaffeensis at an MOI of 20 for 30 h, followed by preservation in RNAlater buffer (Qiagen) at –80 °C for RNA extraction for RT-qPCR analysis. .. To deliver IB mRNA-LNPs in vivo and to examine their effects on Ehrlichia infection, ICR mice (five mice per group; Inotiv, West Lafayette, IN) were intraperitoneally inoculated with E. chaffeensis cultured in THP-1 cells (~ 2 × 10 5 bacteria per mouse), which were preincubated with IB mRNA-LNPs (A44, A123, or A171) or with PBS for 12 h. As E. chaffeensis infects blood monocytes and macrophages, intravenous injection of LNP-mRNAs allows immediate delivery to the bloodstream and rapid systemic distribution to infected cells [ ]. ..

    Article Title: Development of Etf-3-specific nanobodies to prevent Ehrlichia infection and LNP-mRNA delivery in cellular and murine models.
    Article Snippet: Ehrlichia chaffeensis is an obligatory intracellular bacterium that infects monocytes and macrophages and causes human monocytic ehrlichiosis.. Ehrlichia translocated factor-3 (Etf-3) is a type IV secretion system effector that binds host-cell ferritin light chain and induces ferritinophagy, thus increasing cellular labile iron pool for Ehrlichia proliferation.. To further characterize roles of Etf-3 in Ehrlichia infection, we produced immune libraries of Etf-3− specific nanobodies (Nbs).

    Infection:

    Article Title: Development of mRNA–lipid nanoparticle intrabodies against rickettsial infection
    Article Snippet: HEK293 cells (2 × 10 6 cells) were transfected with 2 μg of mRNA-LNPs encoding A44, A123, or A171 or with an equal volume of PBS as a negative control for 6 h. The cells were then infected with host cell–free E. chaffeensis at an MOI of 20 for 30 h, followed by preservation in RNAlater buffer (Qiagen) at –80 °C for RNA extraction for RT-qPCR analysis. .. To deliver IB mRNA-LNPs in vivo and to examine their effects on Ehrlichia infection, ICR mice (five mice per group; Inotiv, West Lafayette, IN) were intraperitoneally inoculated with E. chaffeensis cultured in THP-1 cells (~ 2 × 10 5 bacteria per mouse), which were preincubated with IB mRNA-LNPs (A44, A123, or A171) or with PBS for 12 h. As E. chaffeensis infects blood monocytes and macrophages, intravenous injection of LNP-mRNAs allows immediate delivery to the bloodstream and rapid systemic distribution to infected cells [ ]. ..

    Article Title: Development of Etf-3-specific nanobodies to prevent Ehrlichia infection and LNP-mRNA delivery in cellular and murine models.
    Article Snippet: Ehrlichia chaffeensis is an obligatory intracellular bacterium that infects monocytes and macrophages and causes human monocytic ehrlichiosis.. Ehrlichia translocated factor-3 (Etf-3) is a type IV secretion system effector that binds host-cell ferritin light chain and induces ferritinophagy, thus increasing cellular labile iron pool for Ehrlichia proliferation.. To further characterize roles of Etf-3 in Ehrlichia infection, we produced immune libraries of Etf-3− specific nanobodies (Nbs).

    Cell Culture:

    Article Title: Development of mRNA–lipid nanoparticle intrabodies against rickettsial infection
    Article Snippet: HEK293 cells (2 × 10 6 cells) were transfected with 2 μg of mRNA-LNPs encoding A44, A123, or A171 or with an equal volume of PBS as a negative control for 6 h. The cells were then infected with host cell–free E. chaffeensis at an MOI of 20 for 30 h, followed by preservation in RNAlater buffer (Qiagen) at –80 °C for RNA extraction for RT-qPCR analysis. .. To deliver IB mRNA-LNPs in vivo and to examine their effects on Ehrlichia infection, ICR mice (five mice per group; Inotiv, West Lafayette, IN) were intraperitoneally inoculated with E. chaffeensis cultured in THP-1 cells (~ 2 × 10 5 bacteria per mouse), which were preincubated with IB mRNA-LNPs (A44, A123, or A171) or with PBS for 12 h. As E. chaffeensis infects blood monocytes and macrophages, intravenous injection of LNP-mRNAs allows immediate delivery to the bloodstream and rapid systemic distribution to infected cells [ ]. ..

    Bacteria:

    Article Title: Development of mRNA–lipid nanoparticle intrabodies against rickettsial infection
    Article Snippet: HEK293 cells (2 × 10 6 cells) were transfected with 2 μg of mRNA-LNPs encoding A44, A123, or A171 or with an equal volume of PBS as a negative control for 6 h. The cells were then infected with host cell–free E. chaffeensis at an MOI of 20 for 30 h, followed by preservation in RNAlater buffer (Qiagen) at –80 °C for RNA extraction for RT-qPCR analysis. .. To deliver IB mRNA-LNPs in vivo and to examine their effects on Ehrlichia infection, ICR mice (five mice per group; Inotiv, West Lafayette, IN) were intraperitoneally inoculated with E. chaffeensis cultured in THP-1 cells (~ 2 × 10 5 bacteria per mouse), which were preincubated with IB mRNA-LNPs (A44, A123, or A171) or with PBS for 12 h. As E. chaffeensis infects blood monocytes and macrophages, intravenous injection of LNP-mRNAs allows immediate delivery to the bloodstream and rapid systemic distribution to infected cells [ ]. ..

    Article Title: Development of Etf-3-specific nanobodies to prevent Ehrlichia infection and LNP-mRNA delivery in cellular and murine models.
    Article Snippet: Ehrlichia chaffeensis is an obligatory intracellular bacterium that infects monocytes and macrophages and causes human monocytic ehrlichiosis.. Ehrlichia translocated factor-3 (Etf-3) is a type IV secretion system effector that binds host-cell ferritin light chain and induces ferritinophagy, thus increasing cellular labile iron pool for Ehrlichia proliferation.. To further characterize roles of Etf-3 in Ehrlichia infection, we produced immune libraries of Etf-3− specific nanobodies (Nbs).

    Injection:

    Article Title: Development of mRNA–lipid nanoparticle intrabodies against rickettsial infection
    Article Snippet: HEK293 cells (2 × 10 6 cells) were transfected with 2 μg of mRNA-LNPs encoding A44, A123, or A171 or with an equal volume of PBS as a negative control for 6 h. The cells were then infected with host cell–free E. chaffeensis at an MOI of 20 for 30 h, followed by preservation in RNAlater buffer (Qiagen) at –80 °C for RNA extraction for RT-qPCR analysis. .. To deliver IB mRNA-LNPs in vivo and to examine their effects on Ehrlichia infection, ICR mice (five mice per group; Inotiv, West Lafayette, IN) were intraperitoneally inoculated with E. chaffeensis cultured in THP-1 cells (~ 2 × 10 5 bacteria per mouse), which were preincubated with IB mRNA-LNPs (A44, A123, or A171) or with PBS for 12 h. As E. chaffeensis infects blood monocytes and macrophages, intravenous injection of LNP-mRNAs allows immediate delivery to the bloodstream and rapid systemic distribution to infected cells [ ]. ..

    Article Title: Hybrid neurotoxins and uses thereof
    Article Snippet: .. In short, groups of 5 female ICR mice (Harlan) were injected into the right gastrocnemius muscle with the indicated sub-lethal amounts of toxin in 10 μl of GelPhos buffer (30 mM sodium phosphate [pH 6.3] and 0.2% gelatin) using an insulin syringe. .. The mice's ability to remain running on a Rotarod (MED-Associates) using an accelerating cycle of 4-40 rpm over 5 min was determined daily until full recovery.

    other:


    Article Title: DNA Origami Nanostructures Elicit Dose-Dependent Immunogenicity and Are Nontoxic up to High Doses In Vivo.
    Article Snippet: C. R. Lucas Department of Mechanical and Aerospace Engineering Comprehensive Cancer Center The Ohio State University Columbus Columbus, OH 43210, USA P. D. Halley, A.. A. Chowdury Department of Mechanical and Aerospace Engineering The Ohio State University Columbus, OH 43210, USA B. K. Harrington, J. C. Byrd Comprehensive Cancer Center College of Veterinary Medicine The Ohio State University Columbus, OH 43210, USA

    Negative Control:

    Article Title: Development of Etf-3-specific nanobodies to prevent Ehrlichia infection and LNP-mRNA delivery in cellular and murine models.
    Article Snippet: Ehrlichia chaffeensis is an obligatory intracellular bacterium that infects monocytes and macrophages and causes human monocytic ehrlichiosis.. Ehrlichia translocated factor-3 (Etf-3) is a type IV secretion system effector that binds host-cell ferritin light chain and induces ferritinophagy, thus increasing cellular labile iron pool for Ehrlichia proliferation.. To further characterize roles of Etf-3 in Ehrlichia infection, we produced immune libraries of Etf-3− specific nanobodies (Nbs).



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